A THERMO-RESPONSIVE PNIPAAm-GRAFTED-PDMS SURFACE USED FOR CELL CULTURE IN MICROFLUIDIC CHANNELS
نویسندگان
چکیده
A thermo-responsive PNIPAAm-grafted-PDMS (PNIPAAm-g-PDMS) surface was used for cell culture. Cos7 cells could adhere to the PNIPAAm-g-PDMS surface and proliferated therein at 37 C. The cultured cells could detach from the surface at room temperature without trypsin digestion. The viability of cells harvested from the PNIPAAm-g-PDMS surface via lowering temperature was higher than that of cells harvested from native PDMS surface via trypsin digestion. A PNIPAAm-g-PDMS microchannel was designed for the in-channel thermo-modulated cell culture/harvest. Cells could be long-term cultivated and be harvested without enzymatic treatment. Successful thermo-modulated cell passage operation was demonstrated in the microfluidic chips with the PNIPAAm-gPDMS channels.
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